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nis elements imaging software  (Nikon)
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Nikon nis elements imaging software
A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Nis Elements Imaging Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging software nis element  (Nikon)
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A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Imaging Software Nis Element, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nikon imaging software nis element  (Nikon)
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A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Nikon Imaging Software Nis Element, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Image Processing Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Image Acquisition Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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products software nis elements image studio li cor  (Nikon)
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A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Products Software Nis Elements Image Studio Li Cor, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nikon imaging software elements  (Nikon)
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A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Nikon Imaging Software Elements, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Biofilm

Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

doi: 10.1016/j.bioflm.2025.100335

Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan).

Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software